Italic and boldface type are used for emphasis. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. 303 unable to cal dark current. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Assays vary depending on. 58% for the detection module and 2. The cuvette must be in place when you read the plate. The calculated LOQ values were 0. SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. 314 low lamp level warning. Examples: “Press carefully to engage,” “ Do not. , a. SpectraMax Microplate Readers. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command in SoftMax® Pro. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. B: Selection of Excitation Wavelength. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12The optical system of SpectraMax Plus384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 1000 nm). Compact and tunable absorbance microplate readers DATA SHEET. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Assays can be better optimized by scanning across. Microplate Spectrophotometers. Five preferred detection modes. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Here, we demonstrate performance of the SpectraMax® iD3 Multi-Mode Microplate Reader for assays measuring intrinsic tryptophan fluorescence. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. microplates or in a cuvette at a. The SpectraMax ® M5e Multi-Mode Microplate Reader is a dualmonochromator capable of fluorescence, time-resolved fluorescence (TRF), fluorescence polarization, absorbance, and luminescence assays. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. The temperature in the plate chamber is ambient and isothermal. . Cell Analysis. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. SpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Products. Supplier: Molecular Devices. A: Microplate Preparation. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. On the back, in the right hand top corner,. The CellTiter-Glo assay from Promega uses luciferase. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. 2. The guide also covers the software features, the specifications, and the accessories of the systems. It can run samples based on pre-defined protocols and standard filter modes, and is compatible with ELISA and protein quantification assays. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. The instruments affected are the SpectraMax ® L Microplate Reader and the StakMax® Microplate Handling System. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. Examples: “Press carefully to engage,” “ Do not press down. microplate reader. . with SpectraMax Microplate Readers Introduction Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Microplate Readers. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Published Dec 16, 2020 | Updated Dec 16, 2020. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Figure 1. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Note that this procedure is only for the SpectraMax L model. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path. SpectraMax i3x Multi-Mode Detection Platform. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. AlphaScreen. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Measure more accurately. The SpectraMax L Luminescence Microplate Reader with the variable-volume injectors option (10 µL to 100 µL) can measure both flash and glow luminescence. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. 306 plate air cal fail,low light. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. San Jose, Calif. Category 2 and 3 microplate readers use gradient heating as well. There is the option to add user-installable cartridges as your research needs evolve such as HTRF®,. The chamber contains springs that position the cuvette in the proper alignment for a read. It measures the optical density (OD) of samples in both 96- and 384-well. Additionally, user-installable options allow the SpectraMax i3 System to. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. . Ease of use. no. Compare plate readersFive-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The SpectraMax® iD3 Multi Mode Microplate . The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. Microplate Readers BioTek Epoch 2 Microplate Reader. Introduction. 380 nm/Exc. The Quant-iT PicoGreen dsDNA Assay Kit, when run on a SpectraMax microplate reader with SoftMax Pro Software, is a quick, sensitive detection method for double-stranded DNA. 997 (n = 3). The SpectraMax® ABS Plus Microplate Reader can run . Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Read one sample or up to 384 inThe SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. 72 MB. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. Multimode Microplate Reader. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. ATP standard curves read on SpectraMax microplate readers. Sale. Reporter Assay. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMax® Mini Multi-Mode Microplate Reader for Biotech Laboratories Built on the company’s nearly four decades of experience delivering high-performance microplate reader technology to the market, the SpectraMax Mini reader is robust enough to meet a lab’s core microplate reader needs, while its compact size is ideal for integration into a. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingevery Gemini EM Reader. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. Temperature control. In . 313 reference gain check fail. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Use the provided 3. SpectraMax iD5 Multi-Mode Microplate Reader. 500 Euro/Dollars for a basic filter-based ELISA microplate reader and could go up to over 150. Published Dec 16, 2020 | Updated May 19, 2023. We would like to show you a description here but the site won’t allow us. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. We would like to show you a description here but the site won’t allow us. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. All stand-alone instrument functions can be controlled using the software. Microplate reader assays requiring infrared (IR) or red-shifted. DNA and RNA measurements in SpectraMax® microplateSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. This section contains the following topics:滤光片型光吸收微孔板读板机（酶标仪），简单易用，性价比高，检测结果精确. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Page 28 Chapter 5: Read Modes and Read Types 1. Endpoint, kinetic, spectrum. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. 1. Guides: SpectraMax Mini Installation Guide. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. For SpectraMax ABS Plus reader, r 2 = 0. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Other technologies such as imaging. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. begin reading microplates with seven mouse clicks. Data exemplifying linear ranges from the three different kits are presented in Figure 2. Molecular Devices. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. 76358-624. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. For a higher . The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. In addition, the SpectraMax iD5The SpectraMax® L Luminescence Microplate Reader is a high-performance microplate reader that measures luminescence in 96 and 384-well microplates. It also offers a field-upgradeable cellular imaging option and a cooled PMT for low-light sensitivity. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. . The SpectraMax iD3 reader allows you to interact with your data any way you want, from anywhere you want. Results are shown in Table 1. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Download PDF. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax i3x. Microplate reader settings. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. Other technologies such as imaging. No additional manuals are available. Offer Ends In: 18 Days 19: 38: 21. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD5 reader. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Tecan Infinite Microplate Reader. For readers without a cuvette port, these options are not shown in the software. Or, with the reader specifications noted from the Instrument Description section of your reader's. Here, the CVs for each plate were less than 3, and the difference between mean. Add to Cart. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. View the Molecular Devices website. 2 software: see SoftMax® Pro 7. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. For readers without a cuvette port, these options are not shown in the software. flexibility of monochromator-based systems. e. The Gemini XS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. View your data quickly using the large touchscreen interface, export your data for analysis in the program of. flexibility of monochromator-based systems. 0 out of 5 $ 9,995 $ 9,495. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. both cuvette-based and microplate reader applications on the same instrument. 020 fmol 0. Category 2 and 3 microplate readers use gradient heating as well. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. Keywords: spectramax mini multi-mode microplate reader, spectramax mini, spectramax mini microplate reader Created Date: 5/25/2022 9:57:03 AMFully Warranty. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). Nucleic Acid and Protein Quantitation. Table of Contents. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. Italic and boldface type are used for emphasis. 5 87. With optimized reagents and the industry-leading data acquisition andSpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Figure 6: Transcreener ADP2 FI assay principle. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. When the user applies a set temperature of 37°C, the top is heated to 37. Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. The plate reader optics are tunable for absorbance. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. 5 87. Users can install the ScanLater Western Blot Detection Cartridge in the SpectraMax Paradigm SystemMicroplate readers read biological, chemical or physical events that occur on a microtiter plate. Read one sample or up to 384 in0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The drawing shows a standard microplate of 15 mm height covered with a lid. 7°C (gradient heating design). This is equivalent to having 511 built-in filters, The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. 0 out of 5 $ 11,999. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Results are shown in Table 1. Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. For more sample throughput, both readersSupplier: Molecular Devices. Download PDF versions of the SpectraMax Microplate Reader User Guides. With optimized reagents, option to operate the. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. In addition, the iD5 reader measures TRF and FP. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . zip. The SpectraMax® iD3 and iD5 measure absorbance, fluorescence, and luminescence. 0 out of 5 $ 5,999. Sensitive fluorescent quantitation of DNA with the Quant-iT. limit of detection (LLD) for ATP was estimated for each microplate reader using the . Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. 6% for the on-board optics (monochromators). Path Check Pathlength Measurement SpectraMax i3x. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. For readers without a cuvette port, simply check the box next to ‘PathCheck’. Add to Cart. microplate reader. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. We use Spectramax M5 microplate reader in our lab. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. 305 reference level saturation. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. . In this product video, see how the SpectraMax M5 quickly reads samples. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile microplate reader capable of quantitating samples in a variety of microplate formats. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The SpectraMax Quant dsDNA Assay Kits consist of three products, each designed for a specific range of DNA concentrations and optimized for use with fluorescence microplate readers such as the SpectraMax microplate reader (Table 1). Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. SpectraMax L Microplate ReaderThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and. For biological applications involving small samples,Minimum volume and maximum throughput with 96- or 384-well microplate compatibility. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The plate reader can measure multiple readouts, including UV. Avoid losing high signal. Using the optimized wavelength settings, the SPECTRAmax GEMINI XS fluorescence microplate reader can distinguish between live and dead cells using this assay. \ Figure 3. . Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual monochromators. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. ATP standard curves read on SpectraMax microplate readers. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. The SpectraMax® iD3 multi-mode microplate reader measures absorbance, fluorescence and luminescence and is ideal for busy multi-user, multi-application labs. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Lift up the lid over the chamber to insert or remove a cuvette. 3) This warranty. 7°C and the bottom to 36. The plate reader optics are tunable for. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. 5 12. 0 out of 5 $ 6,499. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. Create custom fluorescence assays without having to purchase expensive filter sets. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. We would like to show you a description here but the site won’t allow us. Offer Ends In: 2 Days 10: 40: 19. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. A preconfigured protocol is available in the software to. Our absorbance plate readers feature our PathCheck Sensor technology and. The system uses two scanning monochromators to determine the optimal excitation and emission settings. This unlocks the transport slide. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 000 Euro/Dollars for a high-end multi-mode microplate reader with several detection modes and the best technology for highest sensitivity and fastest detection. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. We would like to show you a description here but the site won’t allow us. 100 fmol 384-well (75 µL) 0. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. IMAP FP Settings in SoftMax Pro SpectraMax M5 Multi-Mode Microplate Reader. MPN:SpectraMax Plus. Compact designs. , April 14, 2022 – Molecular Devices, LLC. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The Gemini EM can sample a variety of formats, from 6- to 384-well. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. Absorbance (optical density, OD) was read at 570 nm and 605. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. Wide range of applications The VersaMax reader covers. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. ” Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Please refer to the Obsolete Microplate Reader Models for more information. . Spectranet customer portalthe SpectraMax® M Series Multi-Mode Microplate Readers, for . Wide range of applications. edu) to be added as a trained user in iLab. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3 allows users to customize workflow to perfectly. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. If it looks fine, then proceed. . More Product Information. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. . 76358-624. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. The dual monochromator optics allow the widest range of.